Evaluation of the intra-cultivar variation of the Zard olive cultivar (Olea europaea L. CV. Zard) using fatty acids composition and SSR markers

Document Type : Research

Authors

1 Zanjan Agricultural and Natural Resources Research and Education Center

2 Former master's student of Agriculture College of Zanjan University, PhD of Molecular Pathobiology and Genetics Laboratory of Milad Hospital, Tehran, Iran

Abstract

Due to the widespread distribution of the Zard olive cultivar in Iran and its use for double-purpose, it is necessary to study the genetic diversity within this cultivar. To evaluate the amount of variation in the Zard olive (Olea europaea L.) cultivar, using the fatty acids combination and microsatellite markers, an experiment was carried out in 2002 for five years in the Tarom region of Zanjan province. This study used single trees of the Zard cultivar with the codes st1 to st11 and the Zard Golooleh cultivar (st2) as controls. First, the fatty acid composition of the evaluated clones was measured. To extract DNA from young leaves Clones of the Zard olive cultivar were used by the modified CTAB method. Polymerase chain reaction (PCR) was performed by 10 olive-specific markers. To calculate the number of alleles, observed and expected heterozygosity, gene stabilization index, and allele frequency. values, Shannon's information index, similarity probability, and analysis of molecular variance of the data (AMOVA) were used by GeneAlex 6.41 software. The average number of alleles per locus was 3.65, and the highest amount of heterozygosity for the population of Zard clones was equal to one and belongs to five gene locations PUD099-039, PUDO99-007, PUD099-031, PUD099-011 and PssrOelGP7 and the lowest amount of heterozygosity is equal to 0.375 and belonged to the location of PUDO99-036. The changes of fatty acids, especially oleic fatty acid was low among Zard clones. On the other hand, the polymorphisms observed for the clones of the Zard olive cultivar also showed that these clones were very uniform with each other.

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Research in Horticultural Sciences
Volume 1, Issue 2 - Serial Number 2
April 2023
Pages 263-276

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